General Cell Lines and Cell Cultures
Eurofins Discoverx ChemiScreen α2A Adrenergic Receptor Stable Cell Line, 2 vials. (HTS096C)
The ChemiScreen α2A Adrenergic Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. epinephrine and norepinephrine) based activation of ADRA2A GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses ADRA2A alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of ADRA2A, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Revvity Health Sciences Inc cAMPZen Frozen Human Leukotriene BLT1 (LTB4R1) Cell Line, CHO-K1 Cells
cAMPZen Frozen Human Leukotriene BLT1 (LTB4R1) Cell Line, CHO-K1 Cells
Eurofins Discoverx PathHunter SSTR2 Activated GPCR Internalization CHO-K1 Cell Line, 2 vials. (93-0676C2)
The PathHunter SSTR2 Activated GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line uses EFC technology to measure ligand-induced internalization of SSTR2 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged SSTR2. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting SSTR2-arrestin complex is internalized to the early endosome, leading to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Genscript Corporation CHO-K1/VISTA Stable Cell Line
Recombinant CHO-K1 cells stably overexpress V-domain Ig suppressor of T cell activation (VISTA) on the cell surface The surface expression of VISTA is validated by FACS analysis This cell line is designed for cell-based binding for screening antibodies binding with VISTA and evaluating target binding affinity
Amsbio LLC GATA3 Leeporter™ Luciferase Reporter-HEK293 Cell Line
GATA3 Leeporter™ Luciferase Reporter-HEK293 Cell Line
Eurofins Discoverx ChemiScreen ETB Endothelin Receptor Membrane Preparation, 1 vial. (HTS046M)
The ChemiScreen ETB Endothelin Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of EDNRB surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach. Increased binding affinities will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
ATCC Alcanivorax borkumensis
Alcanivorax borkumensis is a whole-genome sequenced bacterial type strain that was isolated in 1991 from sea water sediment in the Isle of Borkum. This strain is cited to degrade n-alkanes and to produce glucose lipids.
ATCC Escherichia coli; Strain CDC EDL 1284
Escherichia coli strain CDC EDL 1284 [929-78] was isolated from human feces. This strain is serotype O124:NM and contains a high-molecular-weight plasmid responsible for enteroinvasiveness.
Eurofins Discoverx PrecisION hKv1.8 Recombinant Stable Cell Line, 2 vials. (CYL3021)
The PrecisION hKv1.8 Recombinant Stable Cell Line is a stable, engineered cell line for use in studying Kv1.8 ion channels, including drug candidates which affect channel function. This cell line enables assessment of ion channel function via detection of electrophysiological signals. The included cell line overexpresses Kv1.8 ion channels. Upon ligand/agonist induced channel activation, response can be detected via automated or manual electrophysiological techniques. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/ion-channels.
Eurofins Discoverx PathHunter U2OS XBP1 Nuclear Translocation Cell Line, 2 vials. (93-0857C3)
The PathHunter U2OS XBP1 Nuclear Translocation Cell Line is a stable, engineered cell line for use in studying drug candidates affecting the IRE1/XBP1 pathway. This cell line enables assessment of ligand (e.g. thapsigargin) based pathway activation via detection of resulting XBP1 nuclear translocation. This product uses EFC technology. The included cell line overexpresses ProLabel-tagged XBP1 and nuclear localized EA. Upon pathway activation, induced nuclear translocation of XBP1 leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/pathways.
Eurofins Discoverx PathHunter PTHR1 Activated GPCR Internalization U2OS Cell Line, 2 vials. (93-0770C3)
The PathHunter PTHR1 Activated GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line uses EFC technology to measure ligand-induced internalization of PTHR1 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged PTHR1. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting PTHR1-arrestin complex is internalized to the early endosome, leading to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter UTR2 Total GPCR Internalization U2OS Cell Line, 2 vials. (93-0746C3)
The PathHunter UTR2 Total GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line uses EFC technology to measure ligand-induced internalization of UTR2 via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged UTR2 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the UTR2 receptor to the early endosome leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Cayman Chemical THUNDRTotal MEK1 TRFRET C 2400
Sandwich TR-FRET immunoassay for semi-quantitative detection of Total MEK1 in cell lysates
Creative Bioarray JHOM-1 Cells, Frozen, 1x10^6 cells/1 mL, Adherent, For Research Use Only
The Creative Bioarray JHOM-1 Cells are a cryopreserved human ovarian epithelial tumor cell line derived from a female patient with mucinous ovarian cancer. Designed specifically for cancer research applications, this adherent cell line provides a valuable in vitro model for studying the mechanisms of ovarian cancer, evaluating drug efficacy, and aiding therapeutic development.
- Derived from a female donor with ovarian epithelial tumor
- Species: Human
- Growth properties: Adherent
- Application: For research use only
- Quantity: 1 x 106 cells / 1.0 ml
- Format: Frozen
- Doubling time: 60 hours
- Available genomic data: Whole exome sequencing, whole genome sequencing
- Available proteomic data: Reverse-phase protein array
- Available transcriptomic data: Microarray, RNAseq
- Included in Cancer Dependency Map and MD Anderson Cell Lines Project